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Uniform TitleDevelopment of a microfluidic module for DNA purification via phenol extraction
NameMorales, Mercedes C. (author), Zahn, Jeffrey (chair), Shinbrot, Troy (internal member), Cai, Li (internal member), Rutgers University, Graduate School - New Brunswick,
Degree Date2008-10
Date Created2008
SubjectBiomedical Engineering,
DNA--Purification,
Microfluidic devices
DescriptionPurification of Deoxyribonucleic acid (DNA) by organic-aqueous liquid extraction, also called phenol extraction, is a standard technique commonly utilized in biology laboratories. In order to minimize interaction energies, membrane components and proteins naturally partition to the organic (phenol) phase while the DNA stays in the aqueous phase, where it can be easily removed. In recent years, microfluidics has become a driving force toward more efficient and autonomous platforms for fluid based diagnostics, chemical reaction chambers, separation and preparation of biological materials.
In this work, the design, fabrication, and performance of long microfluidic devices for DNA extraction are presented. The devices were fabricated using soft lithography to transfer lithographically defined features into a PDMS structure via replica molding. Stratified-flow experiments using a rhodamine dye conjugated bovine serum albumin protein (BSA) in an aqueous phase were conducted to compare different microchannel designs based on their ability to remove proteins from the aqueous phase into the phenol phase. Additionally, the study of BSA partitioning and DNA isolation in a two-phase system under stratified flow condition were addressed, separately and conjunctly. Finally, protein partitioning and DNA recovery were analyzed to evaluate two types of mixing, passive diffusion through stratified flows and droplet enhancement mixing.
In this work, the design, fabrication, and performance of long microfluidic devices for DNA extraction are presented. The devices were fabricated using soft lithography to transfer lithographically defined features into a PDMS structure via replica molding. Stratified-flow experiments using a rhodamine dye conjugated bovine serum albumin protein (BSA) in an aqueous phase were conducted to compare different microchannel designs based on their ability to remove proteins from the aqueous phase into the phenol phase. Additionally, the study of BSA partitioning and DNA isolation in a two-phase system under stratified flow condition were addressed, separately and conjunctly. Finally, protein partitioning and DNA recovery were analyzed to evaluate two types of mixing, passive diffusion through stratified flows and droplet enhancement mixing.
NoteM.S.
NoteIncludes bibliographical references (p. 60-62).
Genretheses
Persistent URLhttp://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.17533
LanguageEnglish
CollectionGraduate School - New Brunswick Electronic Theses and Dissertations
Organization NameRutgers, The State University of New Jersey
RightsThe author owns the copyright to this work.