TitleGeneration and characterization of random and site-directed mutants of Shiga-like toxin 1A by Escherichia Coli O157:H7 in Saccharomyces Cerevisiae
NameShete, Varsha (author), Tumer, Nilgun (chair), Di, Rong (co-chair), Matthews, Karl (internal member), Rutgers University, Graduate School - New Brunswick,
DescriptionFood-borne illnesses are mainly associated with Shiga-like toxins (Stx1 and Stx2) produced by various serotypes of enterohemorrhagic Escherichia coli (EHEC). These serotypes are collectively known as STEC (Stx-producing E. coli). One of these serotypes E. coli O157:H7 has been the major cause of food-borne illnesses recently in US, Canada and Japan. The clinical manifestations of EHEC infections range from watery diarrhea, severe bloody diarrhea, abdominal cramps and hemorrhagic colitis (HC), to the most severe outcome, life-threatening hemolytic uremic syndrome (HUS) resulting in kidney failure.
This study involved generation and characterization of random and site-directed mutants of Shiga-like toxin 1 in Saccharomyces cerevisiae. The mutants were characterized for protein expression, ribosome depurination and loss of cytotoxicity. The results from this study are crucial to understand the mechanism by which Shiga-like toxins exhibit their cytotoxicity to the cells. The results from this study can aid in development of treaments against the diseases caused by Shiga-like toxin 1.
NoteIncludes bibliographical references (p. 33-35)
Noteby Varsha Shete
CollectionGraduate School - New Brunswick Electronic Theses and Dissertations
Organization NameRutgers, The State University of New Jersey
RightsThe author owns the copyright to this work.