TitleStructural studies of cytidine repressor and catabolite activator protein
NameRao, Ramya Rangesh (author), Berman, Helen M (chair), Taylor, John W (internal member), Marcotrigiano, Joseph (internal member), Lawson, Catherine L (outside member), Rutgers University, Graduate School - New Brunswick,
SubjectChemistry and Chemical Biology,
DescriptionThe Escherichia Coli Catabolite Activator Protein (CAP) activates DNA transcription at more than a hundred promoters. Cytidine Repressor (CytR), in conjunction with two CAP dimers, acts as a repressor of DNA transcription at the deoP2 promoter. In the first part of this work, we describe a method by which a (CAP)[subscript] 2-CytR-DNA complex can be prepared for structural studies. In the second part of this project, we describe the crystallization of what was initially
intended to be a (CAP)[subscript] 2-DNA complex in order to study the effects of two CAP dimers on transcription at an artificially constructed promoter containing tandem CAP binding sites. Upon structure determination of the crystal, we observed that while there was no DNA present, the protein had bound multiple Co[superscript]2+ and SO2−/4 ligands. We provide an analysis of the crystal structure and present a possible explanation for the absence of DNA in the structure.
NoteIncludes bibliographical references
Noteby Ramya Rangesh Rao
CollectionGraduate School - New Brunswick Electronic Theses and Dissertations
Organization NameRutgers, The State University of New Jersey
RightsThe author owns the copyright to this work.