TitleGenome-wide screening and physiological responses of saccharomyces cerevisiae to the volatile organic compound 1-octen-3-ol
NamePu, David C. (author), Bennett, Joan W. (chair), Tumer, Nilgun E. (internal member), White, Jr., James F. (internal member), White, Jr., James F. (internal member), Rutgers University, Graduate School - New Brunswick,
Volatile organic compounds--Toxicology ,
DescriptionA major fungal volatile organic compound (VOC) called 1-octen-3-ol or "mushroom alcohol," is produced by most fungal species and is responsible for much of their characteristic musty odor. We believe that fungal VOCs are serving as chemical compounds that signal within a single organism or signal within members of a single species. Also, fungal VOCs may cause “sick building syndrome” (SBS). In this study, we used Saccharomyces cerevisiae as a model for understanding 1-octen-3-ol toxicity on a genome-wide level. A yeast growth assay using solid media prepared in plates sealed with Parafilm was developed. Conditions were identified which permitted reproducible yeast growth measurements when cultures were exposed to 1-octen-3-ol, a challenge due to the volatile nature of the compound. At 48 hours, 300 ppm of 1-octen-3-ol was found to completely inhibit growth of BY4741, a wild type strain. To identify genes and pathways involved in 1-octen-3-ol resistance, we carried out a high-throughput viability assay using the non-essential yeast knockout (KO) library. Of the ~ 4976 strains, we found that 92 (1.8%) showed resistance to 300 ppm of 1-octen-3-ol of which 21 (22.8%) are related to endosome transport. More specifically, 1-octen-3-ol is very likely to inhibit cell grow by the ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway.
NoteIncludes bibliographical references
Noteby David C. Pu
CollectionGraduate School - New Brunswick Electronic Theses and Dissertations
Organization NameRutgers, The State University of New Jersey
RightsThe author owns the copyright to this work.