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TitleThe effect of interleukin 17 on oligodendrocyte proliferation and differentiation
NameTetteh, Sandra (author), Kim, Haesun (chair), Kim, Haesun A (chair), Jonakait, Gene Miller (co-chair), Rutgers University, Graduate School - Newark,
Degree Date2012-10
Date Created2012
SubjectBiology,
Interleukins,
Multiple sclerosis--Research,
Multiple sclerosis--Complications
DescriptionInterleukin-17 (IL-17) is a cytokine that may cross the blood-brain barrier to promote inflammation and participate in the formation of lesions in patients with multiple sclerosis, where recurrent inflammatory attacks lead to oligodendrocyte loss, loss of axons, and ensuing neurological infirmities. Studies have indicated that the levels of IL-17 mRNA are elevated in the cerebrospinal fluid of multiple sclerosis (MS) patients, and microarray analysis of MS lesions show elevated levels of IL-17 transcripts. In this study we set up an in vitro system to examine the role of IL-17 on oligodendrocyte precursor cell (OPC) proliferation and their differentiation into mature oligodendrocytes (OLs). OPCs were treated with increasing doses of IL-17 (0.03 nM, 0.1 nM, 0.3 nM, 0.9 nM) in the presence of PDGF and FGF, and the effect on proliferation was assessed by BrdU uptake. The results show that while IL-17 alone had nominal mitogenic effect on OPCs it promoted growth factor induced proliferation (p<0.0001). Interestingly, the effect on proliferation was only seen at low doses of IL-17, whereas at high doses, the cytokine inhibited OPC proliferation. IL-17 did not have any significant effect on OPC differentiation in culture. All together these results show a dose-dependent effect by IL-17 on OPC proliferation. Additional experiments will be important to further elucidate the role of IL-17 on the oligodendrocyte lineage formation.
NoteM.S.
NoteIncludes bibliographical references
NoteIncludes vita
Noteby Sandra Tetteh
Genretheses
Persistent URLhttp://hdl.rutgers.edu/1782.1/rucore10002600001.ETD.000066589
Languageeng
CollectionGraduate School - Newark Electronic Theses and Dissertations
Organization NameRutgers, The State University of New Jersey
RightsThe author owns the copyright to this work.